OPTIMIZATION OF THE EXTRACTION CONDITIONS OF PHENOLIC COMPOUNDS FROM ALCHEMILLA VULGARIS USING RESPONSE SURFACE METHODOLOGY

The object of this study is to determine the best solvent and optimum extraction conditions for maximum phenolic compounds from Alchemilla vulgaris leaves. Extractions were carried out using solvents with different polarities. Box-Behnken Design was used optimize including time, temperature, liquid/solid ratio. In study, extract obtained acetone-water indicated highest total content (TPC), flavonoid (TFC) antioxidant activity. Optimum both responses found as 168 min, 48.5 °C, ratio 41:1. TPC TFC values determined 7.17 mg GAE/gdw 3.63 QE/gdw under conditions. Concentrations five analyzed by HPLC increased significantl after optimization. results that optimizing critical quantification compounds. present model can contribute food industry where have potential use biopreservatives.